Synergic Inhibition of Lung Carcinoma 95-D Cell Proliferation and Invasion by Combination with (−)-Epigallocatechin-3-Gallate and Ascorbic Acid
MA Yan, YANG Jingya, LI Shiyi, YU Wenjuan1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China; 3. Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China; 4. College of Aqua-Life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
In this study, the anti-invasion effects of (−)-epigallocatechin- 3-gallate (EGCG) mixed with ascorbic acid (Vc) on human lung carcinoma 95-D cells in vitro were examined and the synergism of the combination of EGCG and Vc was evaluated. Soft agar colony formation assay, cell migration assay, invasion assay, western blot analysis of NF-κB, in situ detection of cellular oxidative stress, and statistical analysis were assessed. The results showed that combining EGCG with Vc could inhibit clone forming rate of 95-D cell by 73.2%, reduce the migration ability of 95-D cell by 65.9%, and decrease the intracellular reactive oxygen species(ROS) level by 76.8%. The results of western blot proved that Vc enhanced the activity of EGCG in inhibiting NF-κB localization. It is speculated that the combination of EGCG and Vc can strongly suppress the proliferation and metastasis of lung carcinoma cells in a synergic manner, possibly with a mechanism associated with the scavenging of reactive oxygen species.
Key words:(−)-epigallocatechin-3-gallate (EGCG) reactive oxygen species (ROS) ascorbic acid (Vc) cancer metastasis
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