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武汉大学学报 英文版 | Wuhan University Journal of Natural Sciences
Wan Fang
CNKI
CSCD
Wuhan University
Latest Article
Generation of Linear and Parabolic Concentration Gradients by Using a Christmas Tree-Shaped Microfluidic Network
Time:2018-5-25  
SHEN Qilong, ZHOU Qiongwei, LU Zhigang, ZHANG Nangang
School of Electrical and Electronic Engineering, Wuhan Textile University, Wuhan 430200, Hubei, China
Abstract:
This paper describes a simple method of generating concentration gradients with linear and parabolic profiles by using a Christmas tree-shaped microfluidic network. The microfluidic gradient generator consists of two parts: a Christmas tree-shaped network for gradient generation and a broad microchannel for detection. A two-dimensional model was built to analyze the flow field and the mass transfer in the microfluidic network. The simulating results show that a series of linear and parabolic gradient profiles were generated via adjusting relative flow rate ratios of the two source solutions   and  , which could match well with the experimental results   and  . The proposed method is promising for the genera-tion of linear and parabolic concentration gradient profiles, with the potential in chemical and biological applications such as combinatorial chemistry synthesis, stem cell differentiation or cytotoxicity assays.
Key words:tree-shaped network; concentration gradient; linear profile; parabolic profile
CLC number:O 351
References:
[1]	Dekker L, Segal A. Perspectives: signal transduction. Signals to move cells[J]. Science, 2000, 287(5455): 982-985.
[2]	Parent C A, Devreotes P N. A cell’s sense of direction[J]. Science, 1999, 284(5415): 765-770.
[3]	Weiner O D, Servant G, Welch M D, et al. Spatial control of actin polymerization during neutrophil chemotaxis[J]. Nature Cell Biology, 1999, 1(2): 75-81.
[4]	Walker G M, Sai J, Richmond A, et al. Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator[J]. Lab on a Chip, 2005, 5(6): 611-618.
[5]	Cheng B, Wang S, Chen Y, et al. A combined negative and positive enrichment assay for cancer cells isolation and purification[J]. Technology in Cancer Research & Treatment, 2016, 15(1): 69-76.
[6]	Poulsen C R, Culbertson C T, Jacobson S C, et al. Static and dynamic acute cytotoxicity assays on microfluidic devices[J]. Analytical Chemistry, 2005, 77(2): 667-672.
[7]	Bang H, Lim S, Lee Y, et al. Serial dilution microchip for cytotoxicity test[J]. Journal of Micromechanics & Microengineering, 2004, 14(8): 1165-1170.
[8]	Walker G M, Monteiro-Riviere N, Rouse J, et al. A linear dilution microfluidic device for cytotoxicity assays[J]. Lab on a Chip, 2007, 7(2): 226-232.
[9]	Ye N, Qin J, Shi W, et al. Cell-based high content screening using an integrated microfluidic device[J]. Lab on a Chip, 2007, 7(12): 1696-1704.
[10]	Puttaraksa N, Whitlow H J, Napari M, et al. Development of a microfluidic design for an automatic lab-on-chip opera-tion[J]. Microfluid Nanofluid, 2016, 20(10): 142-152.
[11]	Boyden S. Chemotactic effect of antibody and antigen[J]. Journal of Experimental Medicine, 1962, 115: 453-466.
[12]	Zicha D, Dunn G A, Brown A F. A new direct-viewing chemotaxis chamber[J]. Journal of Cell Science, 1991, 99(4): 769-775.
[13]	Song H J, Poo M M. Signal transduction underlying growth cone guidance by diffusible factors[J]. Current Opinion Neurobiology, 1999, 9(3): 355-363.
[14]	Mao H, Cremer P S, Manson M D. A sensitive, versatile microfluidic assay for bacterial chemotaxis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(9): 5449-5454.
[15]	Walker G M, Ozers M S, Beebe D J. Cell infection within a microfluidic device using virus gradients[J]. Sensors & Actuators B Chemical, 2004, 98(2): 347-355.
[16]	Ketterer S, Hovermann D, Guebeli R J, et al. Transcription factor sensor system for parallel quantification of metabolites on-chip [J]. Analytical Chemistry, 2014, 86(24): 12152-12158.
[17]	Wang W, Cui H, Zhang P, et al. Efficient capture of cancer cells by their replicated surfaces reveals multiscale topo-graphic interactions coupled with molecular recognition[J]. ACS Applied Materials & Interfaces, 2017, 9(12): 10537- 10543.
[18]	Zhou H, Yao S. A facile on-demand droplet microfluidic system for lab-on-a-chip applications[J]. Microfluid Nanofluid, 2013, 16(4): 667-675.
[19]	Jeon N L, Dertinger S K W, Chiu D T, et al. Generation of solution and surface gradients using microfluidic systems[J]. Langmuir, 2000, 16(22): 8311-8316.
[20]	Dertinger S K W, Chiu D T, Jeon N L, et al. Generation of gradients having complex shapes using microfluidic net-works[J]. Analytical Chemistry, 2001, 73(6): 1240-1246.
[21]	Irimia D, Geba D A, Toner M. Universal microfluidic gradient generator [J]. Analytical Chemistry, 2006, 78(10): 3472- 3477.
[22]	Yamada M, Hirano T, Yasuda M, et al. A microfluidic flow distributor generating stepwise concentrations for high- throughput biochemical processing [J]. Lab on a Chip, 2006, 6(2): 179-184.
[23]	Lee K, Kim C, Ahn B, et al. Generalized serial dilution module for monotonic and arbitrary microfluidic gradient generators [J]. Lab on a Chip, 2009, 9(5): 709-717.
[24]	Kim C, Lee K, Kim J H, et al. A serial dilution microfluidic device using a ladder network generating logarithmic or lin-ear concentrations [J]. Lab on a Chip, 2008, 8(3): 473-479.
[25]	Liu W, Lin J M. Online monitoring of Lactate Efflux by multi-channel microfluidic chip-mass spectrometry for rapid Drug Evaluation[J]. ACS Sensors, 2016, 1(4):344-347.
[26]	Gleichmann N, Malsch D, Horbert P, et al. Toward micro-fluidic design automation: A new system simulation toolkit for the in silico evaluation of droplet-based lab-on-a-chip systems[J]. Microfluid Nanofluid, 2014, 18(5): 1095-1105.
[27]	Li Y, Li L, Liu Z, et al. A microfluidic chip of multi-ple-channel array with various oxygen tensions for drug screening [J]. Microfluid Nanofluid, 2016, 20(7): 1-9.
[28]	Duffy D C, McDonald J C, Schueller O J A, et al. Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J]. Analytical Chemistry, 1998, 70(23): 4974-4984.
[29]	Glasgow I, Aubry N. Run with the ball: Sony Entertainment Television changed the way cricket is sold in India, and went on to reinvent the relationship between branding, product placement and programming [J]. Lab on a Chip, 2003, 3(3): 114-120.

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